
WHAT IS TISSUE CULTURE
Tissue culture is the in-vitro aseptic culture of cells, tissues, organs or whole plant under controlled nutritional and environmental conditions conducive for their growth and multiplication.
Plant tissue culture technology is being widely used for large scale plant multiplication, disease elimination, plant improvement and production of secondary metabolites.
TISSUE CULTURE IN AGRICULTURE
STAGE
1
PRODUCTION & PROPAGATION
Large scale propagation of plants
Virus/ disease-free plant cultures
Preservation of rare & endangered species
Embryo rescue
2
STAGE
BREEDING
Somaclonal variation
Haploid & polyploid breeding
Crosses via protoplast fusion
In-vitro hybridization
Faster multiplication of new plantlets
Requires small amount of initial plant tissue
New plantlets and plants are disease and virus-free
Year-round propagation, no seasonality
Development of new subspecies and varieties
WHY TISSUE CULTURE



